HiChIP: efficient and sensitive analysis of protein-directed genome architecture.

Abstract

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

Authors

Mumbach MR  •  Rubin AJ  •  Flynn RA  •  Dai C  •  Khavari PA  •  Greenleaf WJ  •  Chang HY

Link

https://www.ncbi.nlm.nih.gov/pubmed/27643841